CLOSE

Specials

  • March
  • June
  • September
  • Bioanalytical Services
  • Life Science Analytics
  • Life Science Compliance
  • Bioanalytical Services APAC
  • APAC
  • Europe

I agree We use cookies on this website to enhance your user experience. By clicking any link on this page you are giving your consent for us to set cookies. More info

Skip to: Curated Story Group 1
lifesciencesreview
US
APAC
  • Home
  • News
  • Contributors
  • Magazine
  • Conferences
  • Newsletter
  • About

Thank you for Subscribing to Life Science Review Weekly Brief

  • Home
  • News

Recommended picks

Tollys Makes Philippe Goupit as Chairman of the Board

Tollys Makes Philippe Goupit as...

Immudex Releases dCode Dextramer Reagants

Immudex Releases dCode Dextramer...

How Biomarkers Impact Drug Discovery and Development

How Biomarkers Impact Drug Discovery...

Tecan Partners with QIAGEN to Automate Pre-analytical Processing of Blood Samples

Tecan Partners with QIAGEN to...

Clinical Potential of Combining AI and Decentralized Clinical Trials

Clinical Potential of Combining AI...

Proactive Strategies to Keep Container Fleet Intact

Proactive Strategies to Keep...

Improving Access to Quality and Critical Care through Telemedicine

Improving Access to Quality and...

Bioanalytics- A New Ray of Hope

Bioanalytics- A New Ray of Hope

Tollys Makes Philippe Goupit as Chairman of the Board

Tollys Makes Philippe Goupit as...

Immudex Releases dCode Dextramer Reagants

Immudex Releases dCode Dextramer...

How Biomarkers Impact Drug Discovery and Development

How Biomarkers Impact Drug Discovery...

Tecan Partners with QIAGEN to Automate Pre-analytical Processing of Blood Samples

Tecan Partners with QIAGEN to...

Clinical Potential of Combining AI and Decentralized Clinical Trials

Clinical Potential of Combining AI...

Proactive Strategies to Keep Container Fleet Intact

Proactive Strategies to Keep...

Improving Access to Quality and Critical Care through Telemedicine

Improving Access to Quality and...

Bioanalytics- A New Ray of Hope

Bioanalytics- A New Ray of Hope

Why is Liquid Chromatography Coupled with Mass Spectrometry (LC-MS) Favored for Pharmaceutical Bioanalysis?

By Life Sciences Review | Thursday, December 24, 2020
Tweet

Forensic laboratories and doping laboratories perform bioanalysis to detect substance misuse in criminal trials and sports.


FREMONT, CA: Pharmaceutical bioanalysis includes detecting and quantifying drugs in biological fluids such as human blood plasma, serum, whole blood, urine, and saliva. Furthermore, hospital laboratories carry out bioanalysis to ensure if patients are appropriately medicated and compliant.


Bioanalysis is carried out by the pharmaceutical industry during drug production and clinical trials, for pharmacokinetics and toxicokinetics, and ADME (Adsorption, Delivery, Metabolism, Excretion) research linked to new drug substances. Analytical services contract laboratories also perform the above type of measurements. Besides, forensic laboratories and doping laboratories perform bioanalysis to detect substance misuse in criminal trials and sports. Bioanalysis is thus of immense interest to society and is a very active scientific discipline.


Liquid Chromatography, coupled with Mass Spectrometry (LC-MS), is currently the favored instrumental technique for pharmaceutical bioanalysis. However, sample preparation is needed before the LC-MS. Sample preparation may be the dilution of a sample that is typical of urine samples; or Protein Precipitation (PP), Liquid-Liquid Extraction (LLE), Solid-Phase Extraction (SPE). The preparation of the sample aims to make it consistent with the LC-MS system. This occurrence includes removing proteins to prevent contamination of the instrument and removing matrix components that could cause ion suppression, such as phospholipids.


LC–MS-based bioanalytical methods primarily use PP, LLE, or SPE for sample preparation. Since current methods are rooted in these proven sample preparation techniques, which have been developed, perfected, and tested over a long period, acceptance and implementation of new techniques is slow. On the contrary, in scientific literature, sample preparation is an active area of research and development, and micro-extraction is a passionate subject.


In SPME, a thin needle generally coated with Polydimethylsiloxane (PDMS) is placed in the sample solution or headspace, and the target analytes are transferred to this coating. In the next step, the operator or auto-injector inserts the needle into the heated Gas Chromatography (GC) injector. The target analytes thermally desorb and move to the GC column. Just a few years after its release, SPME became commercially available. Since its launch, more than 13,000 SPME-related research papers have appeared, and today scientists are increasingly implementing SPME for routine applications.


Liquid-phase microextraction (LPME) was developed in parallel to SPME. Conceptually, LPME is similar to SPME, except in the former, the extraction process consists of a few microliters of the aqueous solution or organic solvent. Technical structures are referred to as Single-Drop Micro-Extraction (SDME). From SDME, LPME has grown into alternative technological formats like Dispersive Liquid-Liquid Micro-Extraction (DLLME), Hollow-Fiber Liquid-Phase Micro-Extraction (HF-LPME), Dispersive Liquid-Liquid Micro-Extraction Centered on Floating Organic Droplet Solidification (DLLME-SFO), and others.


Check This Out : Top Medical Devices Startups Companies


 


Weekly Brief

loading
Towards a New World Order
> <
  • Other 2021

    Top Vendors

    Current Issue
  • Other 2020

    Top Vendors

    Current Issue

Read Also

Critical Bioanalytical Challenges of ADCs

Critical Bioanalytical Challenges of ADCs

Must-Know Biotechnology Trends in 2021

Must-Know Biotechnology Trends in 2021

Top Biotech Developments to Watch for

Top Biotech Developments to Watch for

Importance of Biomarkers in Drug Development

Importance of Biomarkers in Drug Development
Using AI to Prevent Adverse Effects of Immunotherapy

Using AI to Prevent Adverse Effects of Immunotherapy

Ehave, Inc. Uses Artificial Intelligence And Machine Learning for Big Data Management in Mental Health Care

Ehave, Inc. Uses Artificial Intelligence And Machine Learning for Big Data Management in Mental Health Care

The Role of Technology in the Biotech Sector

The Role of Technology in the Biotech Sector

Why Partner with Bioanalysis Experts?

Why Partner with Bioanalysis Experts?
Loading...

Copyright © 2021 Life Science Review . All rights reserved. |  Subscribe follow on linkedin

This content is copyright protected

However, if you would like to share the information in this article, you may use the link below:

https://www.lifesciencesreview.com/news/why-is-liquid-chromatography-coupled-with-mass-spectrometry-lcms-favored-for-pharmaceutical-bioanalysis-nwid-213.html