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Large-scale whole-genome sequencing is now accessible and practical for the typical researcher because of NGS as it allows scientists to sequence hundreds to tens of thousands of genomes in a single year or study the entire human genome in a single sequencing session.
FREMONT, CA: Next-generation sequencing which is a massively parallel sequencing technique that provides ultra-high throughput, scalability, and speed. The method is used to determine the nucleotide order of entire genomes or specific DNA or RNA portions. NGS has changed biology, allowing labs to undertake a wide range of applications and analyze biological systems at a level never before feasible.
Complex genomics problems nowadays necessitate a level of detail that standard DNA sequencing technology cannot provide. NGS has filled that void and has become a standard tool for addressing these issues.
What Can NGS Do?
NGS technology has fundamentally changed the kinds of questions scientists can ask and answer. A wide range of applications are possible owing to innovative sample preparation and data analysis possibilities. NGS, for example, enables laboratories to:
•Rapidly sequence whole genomes.
• Deeply sequence target regions.
• Discover novel RNA variants and splicing sites using RNA sequencing (RNA-Seq) or quantify mRNAs for gene expression research using RNA-Seq.
• Examine epigenetic variables such as DNA methylation across the genome and DNA-protein interactions.
• Study rare somatic mutations, tumor subclones, and more by sequencing cancer samples.
• Investigate the microbiome of humans.
• Discover new pathogens.
Accessible Whole-Genome Sequencing
The Human Genome Project took over ten years and cost approximately three billion dollars to complete using capillary electrophoresis-based Sanger sequencing. The chain termination method often known as Sanger sequencing, is a method for determining the nucleotide sequence of DNA.
Large-scale whole-genome sequencing is now accessible and practical for the typical researcher because of NGS. It allows scientists to sequence hundreds to tens of thousands of genomes in a single year or study the entire human genome in a single sequencing session.
Next-generation sequencing produces a large amount of DNA sequencing data and is both inexpensive and faster than classic Sanger sequencing.