Consortium for Top-Down Proteomics Announces New Initiative for the CE-MS of Intact Proteins and Protein Complexes

Liangliang Sun of Michigan State University, Alexander R. Ivanov of Northeastern University, and Kevin Jooss of Northwestern University are the initiative's leaders.

FREMONT, CA: The Consortium for Top-Down Proteomics has initiated a new initiative to produce guidelines for using online Capillary Electrophoresis (CE) in top-down and native mass spectrometry to separate proteins and non-covalent protein complexes (MS). The effective separation of mixtures into their constituents before MS analysis is one of the most difficult tasks in analyzing intact proteins and protein complexes. CE's high resolution will be especially useful in differentiating different proteoforms and non-covalent protein complexes found in biological and biopharmaceutical materials.

The Consortium for Top-Down Proteomics is a 501(c)(3) non-profit organization dedicated to fostering collaboration, education, and cutting-edge research to accelerate the thorough study of all human proteoforms, advancing treatments, diagnostics,environment, and energy.

Liangliang Sun of Michigan State University, Alexander R. Ivanov of Northeastern University, and Kevin Jooss of Northwestern University are the initiative's leaders. The Initiative will assess the present state of the art by separating mixtures of proteins and protein complexes with CE and then measuring intact mass and structural fragments online with MS.

The participating laboratories will represent various CE and MS systems, providing an accurate picture of today's capabilities. Based on these findings, the study's leaders will devise a set of recommended experimental conditions and parameters to aid researchers who are combining this powerful separation technology with high-performance mass spectrometry for top-down and native CE-MS analysis of complex biological and biopharmaceutical samples.