AtlasXomics and EpiCypher announce partnership to commercialize spatial epigenomics assays
NEW HAVEN - AtlasXomics and EpiCypher have joined forces to collaborate on the development of CUT&Tag kits and assay services for spatial epigenomics applications. These assays will be designed on AtlasXomics' DBiT-seq platform, utilizing CUT&Tag reagents and antibodies from EpiCypher. This collaboration aims to expand the spatial 'omics toolbox to include histone post-translational modifications (PTMs), transcription factors, and other chromatin regulators.
Spatial technologies play a crucial role in enabling joint analyses of cellular and molecular heterogeneity from intact tissues. Applying ultra-sensitive chromatin mapping assays, such as CUT&Tag, to spatial techniques is essential for studying biological mechanisms in development and disease. CUT&Tag, developed by Dr. Steven Henikoff at Fred Hutchinson Cancer Center and commercialized by EpiCypher, employs a fusion of Tn5 transposase, protein A, and protein G (pAG-Tn5) to cleave and add sequencing adapters at antibody-bound chromatin. Due to its exceptional sensitivity and versatility, CUT&Tag has become the leading platform for single-cell and spatial epigenomics applications.
AtlasXomics' DBiT-seq platform, originally developed by Professor Rong Fan at Yale University, utilizes a combination of microfluidics and next-generation sequencing for high-resolution spatial profiling in tissues. DBiT-seq has gained significant traction in the field, with successful applications in various tissues across more than 50 laboratories worldwide. While AtlasXomics has already commercialized DBiT-seq for spatial ATAC-seq assays to map chromatin accessibility, the partnership with EpiCypher aims to introduce spatial CUT&Tag for profiling chromatin proteins, including histone PTMs and transcription factors.
"By seamlessly integrating molecular biology with histology, the DBiT-seq platform is revolutionizing epigenomics research," stated Colin Ng, Vice President of Business Development at AtlasXomics. "We have developed robust spatial assays that incorporate EpiCypher's proprietary pAG-Tn5 enzyme and high-efficiency antibodies to provide the optimal balance of sensitivity and specificity required for in-depth exploration of histone modifications and chromatin-associated proteins. Together, we will bring spatial CUT&Tag assays to market, including an expanded set of validated antibodies to provide customers with immediate access to diverse critical targets."
EpiCypher holds key rights to CUT&Tag technology and a proprietary nucleosome-based approach to develop ultra-efficient antibodies for spatial and single cell CUT&Tag assays. "Spatial CUT&Tag depends on specific and efficient core reagents to capture more data per cell, which allows deeper biological insights," said Dr. Martis Cowles, Chief Business Officer at EpiCypher. "We are very impressed with the DBiT-seq platform and look forward to working with AtlasXomics to maximize its impact on chromatin science and precision medicine."
AtlasXomics and EpiCypher have focused initial development efforts on fresh frozen samples. Their long-term goal is to optimize spatial CUT&Tag for formalin-fixed paraffin-embedded (FFPE) samples, thereby greatly enhancing the versatility of these assays for clinical applications.
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