CLOSE

Specials

I agree We use cookies on this website to enhance your user experience. By clicking any link on this page you are giving your consent for us to set cookies. More info

Skip to: Curated Story Group 1
lifesciencesreview
US
EUROPE
APAC
CANADA
  • US
    • US
    • EUROPE
    • APAC
    • CANADA
    • LATAM
  • Home
  • Contributors
  • News
  • Conferences
  • Newsletter
  • Whitepapers
  • Magazine
×
#

Life Science Review Weekly Brief

Be first to read the latest tech news, Industry Leader's Insights, and CIO interviews of medium and large enterprises exclusively from Life Science Review

Subscribe

loading

Thank you for Subscribing to Life Science Review Weekly Brief

  • Home
  • News

Recommended picks

How Data Science is Revolutionizing Genomics

How Data Science is Revolutionizing...

Human-specific Proteins Uncovered by Exploring Genetic Uncharted Territory

Human-specific Proteins Uncovered by...

A Broader Perspective of Genomics

A Broader Perspective of Genomics

The Future Of Genomics In Theory-Focused Approaches

The Future Of Genomics In...

Role of Human Genomic Data in Future

Role of Human Genomic Data in Future

Essentiality of Single-cell Genomics Technology

Essentiality of Single-cell Genomics...

Tactics to Handle Genomic Data

Tactics to Handle Genomic Data

Benefits of Advanced Genomic Testing

Benefits of Advanced Genomic Testing

How Data Science is Revolutionizing Genomics

How Data Science is Revolutionizing...

Human-specific Proteins Uncovered by Exploring Genetic Uncharted Territory

Human-specific Proteins Uncovered by...

A Broader Perspective of Genomics

A Broader Perspective of Genomics

The Future Of Genomics In Theory-Focused Approaches

The Future Of Genomics In...

Role of Human Genomic Data in Future

Role of Human Genomic Data in Future

Essentiality of Single-cell Genomics Technology

Essentiality of Single-cell Genomics...

Tactics to Handle Genomic Data

Tactics to Handle Genomic Data

Benefits of Advanced Genomic Testing

Benefits of Advanced Genomic Testing

Types of DNA Sequencing Technologies

Life Sciences Review | Thursday, August 19, 2021
Tweet

Depending on the target, Next-Generation Sequencing (NGS) analysis can range from a few genes to an entire genome. The sequence of DNA bases across the genome and exome is determined by Whole-Genome Sequencing (WGS) and Whole-Exome Sequencing (WES), respectively.


FREMONT, CA: Some techniques have been extinct since the advent of sequencing in the 1970s. In contrast, others have advanced to become the methods of choice for many researchers trying to understand genetic variation today.


Capillary electrophoresis and fragment analysis


Instruments that use Capillary Electrophoresis (CE) can perform both Sanger sequencing and fragment analysis. DNA fragments are fluorescently numbered, separated by CE, and sized by reference to an internal norm of fragment analysis. At the same time, DNA sequencing by CE is used to identify the precise base sequence of a particular fragment or gene segment. This technique can also offer sizing, relative quantitation, and genotyping data for fluorescently labeled DNA fragments produced by PCR employing primers designed for a specific DNA target. DNA fragment analysis can be used for anything from cell line authentication to aneuploidy detection. Although sequencing techniques may also be employed for these applications, researchers prefer fragment analysis because it is more cost-effective and has a quicker turnaround time.


Next-Generation Sequencing (NGS)


While Sanger sequencing has seen numerous advancements over the years, new high-throughput techniques known as next-generation sequencing (NGS) technologies have also emerged. NGS is carried out in a massively parallel manner. Depending on the target, NGS analysis can range from a few genes to an entire genome. The sequence of DNA bases across the genome and exome is determined by Whole-Genome Sequencing (WGS) and Whole-Exome Sequencing (WES), respectively.


To determine differences and gene expression levels across the entire transcriptome, whole-transcriptome sequencing provides sequence information about coding and multiple noncoding types of RNA. Targeted sequencing focuses on a limited number of genes or regions of interest. Targeted sequencing is well suited for both translational and clinical research applications due to its fast turnaround time, low cost, low sample input requirement, and relative ease of interpretation. Sanger sequencing is often used to validate variants discovered using NGS. Sanger sequencing, fragment analysis, and NGS allow a slew of cutting-edge applications that advance the understanding of genomes.


Weekly Brief

loading
Top 10 Genomics Solutions Companies in Europe/UK – 2022
> <
  • Clinical Lab Equipment 2023

    Top Vendors

    Current Issue
  • Proteomics 2022

    Top Vendors

    Current Issue
  • Clinical Lab Equipment 2023

    Top Vendors

    Current Issue
  • Proteomics 2022

    Top Vendors

    Current Issue

Read Also

Qualities to Consider when Choosing Life Sciences Business Consultants for Your Firm

How Biotech Startups can Mitigate Risks in Order to Grow Sustainably

Cellf BIO's BioShincter to Undergo Phase 1 Trail to Test Fecal Incontinence Treatment

Fulcrum Therapeutics Announces CEO Transition

An Overview of Wholesalers' Roles in Complex Supply Chains

Veeva Opening Up Integration Module for Future Opportunities and Applications

Veeva Systems To Accelerate the CRM of Life Science Businesses

The Role of Technology in Advancing Clinical Trials

Loading...

Copyright © 2023 Life Sciences Review . All rights reserved. |  Subscribe |  About Us follow on linkedin

This content is copyright protected

However, if you would like to share the information in this article, you may use the link below:

https://www.lifesciencesreview.com/news/types-of-dna-sequencing-technologies-nwid-373.html